Figure 3: nc-tgp1, not nc-1343, represses tgp1+ to confer drug tolerance. | Nature Communications

Figure 3: nc-tgp1, not nc-1343, represses tgp1+ to confer drug tolerance.

From: Long non-coding RNA-mediated transcriptional interference of a permease gene confers drug tolerance in fission yeast

Figure 3

(a) Schematic diagram indicating strategic manipulations of lncRNAs upstream of tgp1+ including 1343Δ, shorter deletions of ncRNA.1343 (AΔ and BΔ) and ura4+ integration within the nc-tgp1 lncRNA locus (nc-tgp1:ura4+) in wild-type background. (b) Serial dilutions of wild-type, 1343Δ, AΔ, BΔ and nc-tgp1:ura4+ were spotted on non-selective YES medium or in the presence of TBZ (20 μg ml−1), HU (10 mM) or caffeine (15 mM), respectively. (c) RT–qPCR experiments measured tgp1+, nc-tgp1 and nc-1343 transcript levels in wild-type, 1343Δ, AΔ, BΔ and nc-tgp1:ura4+ cells. Error bars represent s.e.m. resulting from three independent replicates.

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