Figure 4: Phosphate starvation induces tgp1⁺ and reduces lncRNA transcription.

(a) RT–qPCR experiments measured tgp1⁺, nc-tgp1 and nc-1343 transcript levels in wild-type cells grown in phosphate-rich medium (+PO₄) or in the absence of phosphate (−PO₄). pho1⁺ is a positive control for phosphate starvation. (b) Northern analysis of tgp1⁺ in wild-type cells grown in the presence or absence of phosphate and 1343Δ grown in the presence of phosphate. (c) Rbp1 ChIP–qPCR experiments performed in wild-type cells grown in the presence or absence of phosphate and 1343Δ grown in the presence of phosphate. Error bars represent s.e.m. resulting from three independent replicates.