Figure 2: Loss of PHD3 increases tumour growth. | Nature Communications

Figure 2: Loss of PHD3 increases tumour growth.

From: Loss of PHD3 allows tumours to overcome hypoxic growth inhibition and sustain proliferation through EGFR

Figure 2

(a) Immunoblot of G55 glioblastoma cells stably transduced with control (co), PHD2 or PHD3 shRNA following exposure to 21% (N) or 1% O2 (H) for 24 h. (b,c) PHD3, but not PHD2, loss promotes intracranial glioma growth. Tumour xenografts of polyclonal G55 pools expressing control, PHD2, PHD3 or PHD2/PHD3 shRNA were stained with haematoxylin and eosin (HE; n=8). (d) Kaplan–Meier survival curves of nude mice intracranially injected with polyclonal G55 pools expressing control or PHD3 shRNA. (e) Immunoblot of wild-type (WT) and PHD3−/− astrocytoma cells following exposure to 21% (N) or 1% O2 (H) for 24 h. (f,g) Genetic inactivation of PHD3 increases mouse astrocytoma growth (n=9–10). (h,i) PHD3 silencing reduces tumour cell apoptosis in xenografts of polyclonal G55 pools (n=7–8) assessed by quantifying the number of TUNEL-positive cells per tumour area. (j,k) Silencing of PHD3 increases cell proliferation in xenografts of polyclonal G55 pools (n=8) quantified as the number of phospho-histone 3-positive cells per tumour area. Western blots images (a,e) have been cropped for presentation. Full size images are presented in Supplementary Fig. 10. All values are means+s.e.m., *P<0.05; **P<0.01; ***P<0.001. Scale bars, 1 mm (b,f), 50 μm (h,j).

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