Figure 3: Loss of PHD3 promotes tumour cell survival and proliferation. | Nature Communications

Figure 3: Loss of PHD3 promotes tumour cell survival and proliferation.

From: Loss of PHD3 allows tumours to overcome hypoxic growth inhibition and sustain proliferation through EGFR

Figure 3

(a,b) PHD3 silencing increases clonal cell growth in a 3D spheroid culture system. G55 cells expressing control or PHD3 shRNA were cultured as spheroids in B27-supplemented serum-free medium±EGF/FGF and the number of spheroids was quantified after 3 days (n=6). (c,d) PHD3 loss protects against cell death induction following growth factor withdrawal. Apoptosis was assessed by quantifying the number of TUNEL-positive cells after 48 h of incubation in B27-supplemented serum-free medium±EGF/FGF (n=15). (e,f) PHD3 loss increases cell proliferation. Cell proliferation was assessed by quantifying the number of 5-bromodeoxyuridine-positive cells after 48 h of incubation in B27-supplemented serum-free medium±EGF/FGF (n=15). (g) PHD3 disruption by genetic inactivation confers a growth advantage in additional glioma cell systems. Clonal cell growth was quantified as in b in the presence or absence of EGF/FGF in PHD3−/− astrocytoma cells (n=6). All values are means+s.e.m., **P<0.01; ***P<0.001. Scale bars, 50 μm.

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