Figure 3: FGF signalling is active in somitic tissues but not in the pre- endothelial PLM during mid-somitogenesis. | Nature Communications

Figure 3: FGF signalling is active in somitic tissues but not in the pre- endothelial PLM during mid-somitogenesis.

From: FGF signalling specifies haematopoietic stem cells through its regulation of somitic Notch signalling

Figure 3

(a–c) Images of double enzymatic WISH using probes for the FGF target gene erm (a), fgfr1 (b), and fgfr4 (c) expressed in somites (black arrows) and the PLM marker fli1 (red arrows) at 15 hpf. (d–l) Confocal images of two-colour FISH with the PLM marker lmo2 (green in d,g,j), fgfr1, fgfr4 and the FGF target gene pea3 (red in e,h,k) and merged images (f,i,l). (m,n) qPCR expression of pre-endothelial PLM fli1:EGFP+ cells and somite-specific α-actin:GFP+ cells at 17 hpf. Expression data were normalized to ef1α levels (α-actin+ fraction, n=3; *P value<0.005, significantly different from fli1+, Student’s t-test). Scale bar=100 μm.

Back to article page