Figure 6: FGF regulation of HSC specification requires the Notch ligand Dlc. | Nature Communications

Figure 6: FGF regulation of HSC specification requires the Notch ligand Dlc.

From: FGF signalling specifies haematopoietic stem cells through its regulation of somitic Notch signalling

Figure 6

(a–d) Expression of runx1 in wt and hsp70:dn-fgfr1 embryos injected with 100 pg of dlc mRNA and uninjected controls. Embryos were heat induced at 12 hpf. (e,f) Quantitation of defective embryos showing decreased expression of runx1 at 26 hpf (e; uninjected wt, n=61; dlc+wt, n=44; uninjected hsp70:dn-fgfr1, n=46; dlc+hsp70:dn-fgfr1, n=52) and cmyb+ cells at 32 hpf (f; uninjected wt, n=68; dlc+wt, n=56; uninjected hsp70:dn-fgfr1, n=58; dlc+hsp70:dn-fgfr1, n=46) using hsp70:dn-fgfr1 injected with dlc mRNA and followed by heat-shock at 12 hpf (mean±s.e.m.; *P<0.05, Student’s t-test). (g–j) runx1 expression at 26 hpf in hsp70:gal4; UAS:NICD-myc; hsp70:dn-fgfr1 heat-shocked at both 12 and 14 hpf. (k,l) NICD+ embryos were identified by immunostaining for the myc epitope tag, following WISH. (m) Percentage of embryos with a runx1 phenotype at 26 hpf in wt or FGF-blocked, with or without enforced NICD expression (NICD- wt, n=48; NICD+ wt, n=25; NICD- hsp70:dn-fgfr1, n=20; NICD+ hsp70:dn-fgfr1, n=21). Red arrowheads indicate runx1 expression in the DA. Scale bar=100 μm.

Back to article page