Figure 2: Aurora kinase B is required for MeCP2 S421 phosphorylation in the aNPCs. | Nature Communications

Figure 2: Aurora kinase B is required for MeCP2 S421 phosphorylation in the aNPCs.

From: Cell cycle-linked MeCP2 phosphorylation modulates adult neurogenesis involving the Notch signalling pathway

Figure 2

(a,b) Western blot analysis and quantification of the relative MeCP2 S421 phosphorylation level in aNPCs: (1) dimethyl sulphoxide (DMSO), (2) 36 h of nocodazole (150 ng ml−1) treatment, (3) 24 h of hesperadin (2 μM) treatment after pre-synchronization of the cells by nocodazole for 12 h. (n=3 in each group). (c,d) Western blot analysis and quantification of the relative MeCP2 S421 phosphorylation level in EGFP-shRNA or AURKB-shRNA lentivirus-infected aNPCs, which are treated with DMSO or nocodazole for 24 h. (e,f) Western blot analysis reveals that MeCP2 and AURKB are co-immunoprecipitated reciprocally. (g) Western blot analysis reveals endogenous interaction between MeCP2 and AURKB in aNPCs. (h) In vitro kinase assay followed by western blotting demonstrates that AURKB can phosphorylate S421 on MeCP2. Numbers next to western blottings are molecular weight markers.

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