Figure 4: Adhesion frequency assay.
From: Ligand-engaged TCR is triggered by Lck not associated with CD8 coreceptor
(a) Frequency of adhesion was normalized to the amount of pMHC presented (<n>/mpMHC) and plotted against time t for OT-I hybridomas expressing CD8α wild-type and CD8β-wild-type (closed circles), or CD8α-CxCPmut and CD8β wild-type (closed squares) T cells interacting with RBCs bearing 4 and 7 OVA:H-2Kb per mm2, respectively, at 37 °C. Controls with CD8 wild-type cells pretreated with a blocking antibody against TCR (open diamond), or using RBCs bearing 93 VSV:H-2Kb per mm2 (open triangle) were conducted. Representative data from three repeated experiments are shown. (b) <n>/mpMHC versus t data of OT-I hybridomas expressing CD8α wild-type and CD8β-MHCmut (closed triangles) T cells interacting with RBCs bearing 23 OVA:H-2Kb per μm2. Controls with CD8 wild-type cells pretreated with a blocking antibody against TCR (open diamond), or using RBCs bearing 93 VSV:H-2Kb per mm2 (open triangle) were conducted. Data are representative of three experiments.
