Figure 6: Src kinase family activity is responsible of TCR phosphorylation after antigen recognition.
From: Ligand-engaged TCR is triggered by Lck not associated with CD8 coreceptor
Primary OT-I CTL were pretreated or not with the general Src kinase inhibitor PP2 (20 mM) and then stimulated with either H2-Kb-OVA or H2-Kb-OVA-HLA-A*02:01 tetramers for the indicated times. CD3ζ phosphorylation was analysed by immunoblot. Quantification of band intensities is shown in the left plot. In the right-hand plot, cells were loaded with Ca2+-sensitive dye Fluo-4 and imaged after the addition on top of bilayers containing either H2-Kb-OVA or H2-Kb-OVA-HLA-A*02:01 monomers plus ICAM-1, as described in the Methods section. Data are representative of four experiments. Data are representative of three experiments.
