Figure 2: Experimental SMFS unfolding traces.
From: Ultrastable cellulosome-adhesion complex tightens under load
(a) Unfolding fingerprints from pulling configuration I (curves 1 & 2) and configuration II (curve 3). The QM-FRC model (dashed lines) was used to estimate the contour lengths of the unfolded modules. (b) Contour length histogram obtained from 127 force extension traces (Config. I). The peak-to-peak increments correspond to Xyn, CBM and XMod amino-acid sequence lengths. (c) Dynamic force spectra for the final Doc:Coh complex rupture peaks obtained from 2,122 force-extension traces. The blue points show Doc:Coh ruptures that occurred with an intact XMod, while grey points show ruptures immediately following XMod unfolding. Black circles and diamonds represent the most probable rupture force/loading rate obtained by Gaussian fitting at each pulling speed. Error bars are ±1 s.d. Dashed lines are least square fits to the Bell-Evans model.
