Figure 1: Inverse correlation between Mcl-1 and Beclin 1 levels. | Nature Communications

Figure 1: Inverse correlation between Mcl-1 and Beclin 1 levels.

From: Beclin 1 restrains tumorigenesis through Mcl-1 destabilization in an autophagy-independent reciprocal manner

Figure 1

(a) Assessment of autophagy markers in 293T cells transfected with the indicated siRNAs and grown in nutrient-rich medium. (b) Mcl-1 knockdown in 293T cells transfected with tetracycline-inducible shRNA against Mcl-1 and either treated with Doxycycline (100 ng ml−1) or left untreated for the indicated time points. (c) Pharmacological depletion of Mcl-1 in 293T cells treated with sorafenib (10 μM), roscovitine (25 μM) or ABT 737 (1 μM) as control for the indicated time points. (d) Stabilization of endogenous Mcl-1 and concurrent decrease in Beclin 1 in 293T cells transfected with the indicated siRNAs. (e) Mcl-1 upregulation upon Beclin 1 knockdown in 293T cells transfected with the indicated shRNAs.

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