Figure 2: Reciprocal regulation between Mcl-1 and Beclin 1 protein stability and ubiquitination. | Nature Communications

Figure 2: Reciprocal regulation between Mcl-1 and Beclin 1 protein stability and ubiquitination.

From: Beclin 1 restrains tumorigenesis through Mcl-1 destabilization in an autophagy-independent reciprocal manner

Figure 2

Beclin 1 (a) and Mcl-1 (b) mRNA levels in 293T cells transfected with the indicated siRNAs/shRNAs. Results are representative of at least three independent experiments. Error bars represent s.e.m. (c,d) Beclin 1 and Mcl-1 protein levels in 293T cells transfected with the indicated siRNAs/shRNAs and treated with or without cycloheximide (20 μg ml−1) for 6 h. (e,f) Mcl-1 and Beclin 1 protein levels in 293T cells treated with the inhibitors of either the lysosomal degradation pathway (100 nM Bafilomycin A, 10 μg/ml E64d+Pep) or the proteasome (2.5 μM MG132, 25 nM Epoximicin) for the indicated time points. (g,h) Beclin 1 or Mcl-1 were immunoprecipitated from 293T cells transfected with the indicated siRNAs/shRNAs and assessed for their ubiquitination levels.

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