Figure 4: Dynamin recruitment on membranes depends on BIN1-induced PtdIns(4,5)P2 clustering. | Nature Communications

Figure 4: Dynamin recruitment on membranes depends on BIN1-induced PtdIns(4,5)P2 clustering.

From: BIN1/M-Amphiphysin2 induces clustering of phosphoinositides to recruit its downstream partner dynamin

Figure 4

(a,b) Binding of 0.3 μM dynamin (green) to flat membranes containing 5% PtdIns(4,5)P2 either displaying lipid tubes (white arrows, left panel) or not (right panel) after BIN1 injection (cyan). (c) Binding of 0.1 μM dynamin to flat membranes containing 5% PtdIns(4,5)P2 after BIN1 injection. Measurements were performed from at least three independent experiments (n=15, n=24 and n=13, for 0.3 μM dynamin in control conditons, +lipid tubes, and −lipid tubes, respectively. n=13 and n=21 for 0.1 μM dynamin in control conditons and+BIN1, respectively). Box plots display the average value (solid square, also shown on top of each box plot), the median value (solid line), the s.d. and the interquartile range (25–75%). Error bars represent s.d. ***P<0.001 (t-test). Error bars represent s.d.. Scale bars, 20 μm.

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