Figure 2: Mms4 and Yen1 compensate one another in resolving chromosomes XII and IV sister chromatids DNA-DNA linkages on ongoing DNA damage. | Nature Communications

Figure 2: Mms4 and Yen1 compensate one another in resolving chromosomes XII and IV sister chromatids DNA-DNA linkages on ongoing DNA damage.

From: Mus81-Mms4 and Yen1 resolve a novel anaphase bridge formed by noncanonical Holliday junctions

Figure 2

Strains FM588 (cdc15-2 Tel-cXIIr:tetO tetR-YFP) and its derivatives carrying either mms4Δ or yen1Δ, plus the double mutant mms4Δ yen1Δ, were treated as in Fig. 1. (a) Pulsed-field gel electrophoresis (PFGE) of all yeast chromosomes at the cdc15-2 telophase block. Left picture: gel after ethidium bromide staining. Right picture: Southern blot of that gel against an rDNA probe, that is, cXII. (b) Quantitation of chromosome bands for XII, IV, XVI+XIII and IX relative to the V+VIII chromosome band intensity. The plot is from the gel shown plus two more independent experiments (mean±s.e.m., N=3). The minus and plus signs represent the absence of MMS or the presence of 0.004% (v/v) MMS in the cultures, respectively.

Back to article page