Figure 1: miR-378/378* overexpression dysregulates glucose/lipid homeostasis. | Nature Communications

Figure 1: miR-378/378* overexpression dysregulates glucose/lipid homeostasis.

From: Hepatic miR-378 targets p110α and controls glucose and lipid homeostasis by modulating hepatic insulin signalling

Figure 1

(a) The levels of miR-378/378* in the liver of fed, fasted and refed mice (n=5). Means±s.e.m. are shown. **P<0.01 versus fed mice; #P<0.05 versus fasted mice; ##P<0.01 versus fasted mice. (b) Time course of random glucose levels of mice after Ad-null or Ad-378 infection (n=9-10). Blood glucose levels (c, n=15–17) and insulin levels (d, n=8–9) of mice infected with Ad-null or Ad-378 were examined under different feeding conditions. (e–g) Glucose-tolerance test (GTT; e, n=6–7), insulin-tolerance test (ITT; f, n=9–10) and pyruvate-tolerance test (PTT) (g, n=9–10) were performed in mice infected with Ad-null or Ad-378. Area under the curve (AUC) data for GTT (e), ITT (f) and PTT (g) tests were calculated, respectively. The expression of G6Pase, PEPCK, PGC-1α and SHP in the liver of mice (h, n=5) or mouse primary hepatocytes (i, n=3) infected with Ad-null or Ad-378 as indicated. PAS staining (j) and glycogen assay (k, n=7) were carried out to determine the glycogen storage in the liver of mice infected with Ad-null or Ad-378. Representative results of PAS staining were shown (j). Scale bar, 50 μm. The amount of glycogen was normalized based on protein weight (k). (l) The ratio of liver weight to body weight (LW/BW) was determined in mice infected with Ad-null or Ad-378 (n=10-11). (m) Liver and serum TG levels were measured in random fed state (n=10-11). (n) Representative images of H&E staining (left) and Oil-red O staining (right) of liver sections from ad libitum-fed mice infected with Ad-null (top) or Ad-378 (bottom). Scale bar, 50 μm. Image-Pro Plus was employed for quantification of lipid content. (o) Relative mRNA levels of ACC1, FASN, SCD1 and GCK in the liver of mice infected with Ad-null or Ad-378 (n=5). (p) Protein levels of ACC and FSAN were determined by western blot analysis and quantified. Each mouse received 1 × 109 PFU of adenovirus. Means±s.e.m. are shown for all panels. *P<0.05 versus control; **P<0.01 versus control; ***P<0.001 versus control (Student’s t-test). All experiments were repeated at least twice and representative results are shown.

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