Figure 2: Hepatic overexpression of miR-378/378* impairs insulin signalling. | Nature Communications

Figure 2: Hepatic overexpression of miR-378/378* impairs insulin signalling.

From: Hepatic miR-378 targets p110α and controls glucose and lipid homeostasis by modulating hepatic insulin signalling

Figure 2

Western blot analysis of phosphorylated key molecules of insulin/PI3K pathway in the liver of mice infected with Ad-null or Ad-378 (1 × 109 PFU per mouse) after insulin administration (a, n=4–8) or during the fasting-refeeding transition (b, n=5–7). (c) Western blot analysis of phospho-Akt in insulin-stimulated primary hepatocytes infected with Ad-null or Ad-378. (d) Insulin-induced endogenous FoxO1 translocation was visualized using confocal microscopy. Scale bar, 20 μm. (e) Confocal microscopy data were analysed to quantify the ratio of cytoplasmic FOXO1 to total FOXO1 in primary hepatocytes, with or without insulin treatment (n=3). (f) The level of nuclear FOXO1 in the liver of refed mice infected with Ad-null or Ad-378 (1 × 109 PFU per mouse) was determined by western blot analysis and quantified. (g) Insulin-stimulated phosphorylation of Akt was analysed in mouse primary hepatocytes transfected with synthetic miRNA mimics for miR-378 or miR-378*. All experiments were repeated at least twice and representative results are shown.

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