Figure 5: Restoration of p110α abolishes the metabolic action of miR-378. | Nature Communications

Figure 5: Restoration of p110α abolishes the metabolic action of miR-378.

From: Hepatic miR-378 targets p110α and controls glucose and lipid homeostasis by modulating hepatic insulin signalling

Figure 5

(a) Western blot and densitometry analysis of p110α in the liver of mice infected with Ad-378 or both Ad-378 and Ad-p110α. Glucose levels (b), insulin levels (c), the ratio of liver weight to body weight (d), liver TG levels (e) and serum TG levels (f) in mice infected with Ad-378 or both Ad-378 and Ad-p110α (n=8–9). Relative mRNA expression of lipogenic genes (g, ACC1, FASN and SCD1) and gluconeogenic genes (h, G6pase and PEPCK) in the liver of mice infected with Ad-378 or both Ad-378 and Ad-p110α (n=5). (i) Western blot analysis of phosphorylated key molecules in insulin/PI3K signalling and p110α in primary hepatocytes infected with Ad-378 or Ad-p110α, or both Ad-378 and Ad-p110α after insulin administration, as indicated. Means±s.e.m. are shown. *P<0.05 and **P<0.01 (versus control group infected with Ad-null and Ad-GFP) (Student’s t-test); #P<0.05 and ##P<0.01 (versus the group infected with Ad-378 and Ad-GFP) (Student’s t-test). All experiments were repeated twice and representative results are shown.

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