Figure 7: Increasing miR-378/378* expression ameliorates steatosis in ob/ob mice. | Nature Communications

Figure 7: Increasing miR-378/378* expression ameliorates steatosis in ob/ob mice.

From: Hepatic miR-378 targets p110α and controls glucose and lipid homeostasis by modulating hepatic insulin signalling

Figure 7

(a) The ratio of liver weight to body weight (LW/BW) was determined in ob/ob mice infected with Ad-null or Ad-378 (n=4). Liver (b) and serum (c) TG levels were measured for these mice in random fed state (n=4). (d) Fasting glucose level was also determined (n=4). (e) Representative images of H&E staining (left) and Oil-red O staining (right) of liver sections from ad libitum-fed ob/ob mice infected with Ad-null (top) or Ad-378 (bottom). Scale bar, 100 μm. The lipid content was quantified by Image-Pro Plus (right). (f) The protein levels of p110α and FASN. The mRNA expression of lipogenic genes (g, ACC1, FASN, SCD1 and GCK) and gluconeogenic genes (h, PEPCK and G6Pase) was determined by quantitative reverse transcriptase–PCR. (i) Western blot analysis of p110α, phospho-Akt, phospho-FOXO1 and phospho-GSK3β in the liver of Ad-null or Ad-378-infected ob/ob mice after insulin treatment. Means±s.e.m. are shown. *P<0.05, **P<0.01 and ***P<0.001 (Student’s t-test). These experiments were performed at least twice and representative results are shown. (j) Schematic diagram of the working model of hepatic miR-378 in metabolic regulation.

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