Table 3 Effects of FXN and ISCU on the multiple turnover kinetic parameters.
From: Mammalian frataxin directly enhances sulfur transfer of NFS1 persulfide to both ISCU and free thiols
Proteins and complexes | Reductant |
|
|---|---|---|
NI | L-cys | 6.1±0.5 |
NI+F | L-cys | 6.2±0.5 |
NIU | L-cys | 1.2±0.2 |
NIUF | L-cys | 7.1±0.5 |
NI | DTT | 5.3±0.5 |
NI+F | DTT | 5.0±0.5 |
NIU | DTT | 0.6±0.2 |
NIUF | DTT | 12±1 |
(M−1 s−1)- DTT, dithiothreitol; FXN, frataxin; L-cys, L-cysteine.
- The bimolecular rate constants of NFS1 persulfide reduction,
, were determined from multiple-turnover kinetics by linear regression of the initial velocities of L-alanine formation plotted as a function of initial reductant concentrations.
, were determined from multiple-turnover kinetics by linear regression of the initial velocities of 