Figure 3: Native 3D ultrastructure of the 96-nm axonemal repeat in normal human cilia.
From: Cryo-electron tomography reveals ciliary defects underlying human RSPH1 primary ciliary dyskinesia
(a,b) Isosurface renderings show cross-sectional (a) and longitudinal-front (b) views of the averaged 96-nm repeat from 12 tomograms of axonemes. (c–f) Tomographic slice (c; 1-nm-thick) and isosurface rendering (d) of β-ODA from the averaged 96-nm repeat; note major dynein domains: tail (pink), linker (magenta), head (green) and coiled-coil stalk (arrowheads). A diagram (f) shows the domain organization of dynein, including the microtubule-binding domain (MTBD). (g,h) Isosurface rendering (g) and tomographic slice (h; 1-nm-thick) show the I1 dynein (pink) and N-DRC (yellow); note ultrastructural details, such as the I1 stalk (arrowhead). (i) Resolution of the averaged axonemal repeat (Fourier shell correlation=0.5). (j) Isosurface rendering shows a longitudinal back view of the averaged 96-nm repeat. Scale bars: (c) 10 nm, (h) 20 nm.
