Figure 3: Chronic-active G9a interacts with select HMTases during ET. | Nature Communications

Figure 3: Chronic-active G9a interacts with select HMTases during ET.

From: A chromatin activity-based chemoproteomic approach reveals a transcriptional repressome for gene-specific silencing

Figure 3

NSD3 (a) and ASH2L (d) specifically interact with chronically active G9a in LPS-tolerant 293-TLR4/CD14/MD2 cells. Cell responses were monitored by p65 phosphorylation. (b) Lysine methylation at multiple sites on H3 was compared in paired wild-type or G9a KD Raw264.7 cells (left) and in BMDMs with or without 2-day UNC0638 treatment (right). Unmodified H3 was used as the loading control. (c) ChIP assay of NSD3 deposition on IL6 ‘Cc’ and ‘Ee’ region in the same sample pairs as (b). *P<0.05; **P<0.01. Two-tailed unpaired Student’s t-test. (e) Immunoblot analysis of the pulled down products from a paired wild-type versus G9a KD Raw264.7 cells under TL using histone H3 lysine 4 methylated peptides.

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