Figure 6: Chronically active G9a stabilizes c-Myc and promotes cell survival.

(a) c-Myc protein expression was diminished in G9a KD cells under TL conditions. The abundance of G9a was measured to determine G9a knockdown efficiency and β-Actin was the loading control. (b) c-Myc mRNA expression was elevated in G9a KD, LPS-tolerant cells, represented by the relative expression normalized to GAPDH. (c) c-Myc half-life was measured in wild-type (EV) and G9a KD tolerant cells treated with cycloheximide (CHX) in a time-dependent manner. The intensities of c-Myc are shown as y value in the scatter plot, and c-Myc half-life was indicated by crossing dashed line with x axis. γ-Tubulin is the loading control. (d) c-Myc accumulation was investigated by applying a time-course MG-132 treatment on LPS-tolerant cells. (e) Activated G9a is required to maintain cell survival in tolerant cells. Cell viability was measured as survival percentage normalized to non-treated naïve cells. **P<0.01. Two-tailed unpaired Student’s t-test.