Figure 1: Imaging set-up and representative images of the microvascular structures and oxygenation. | Nature Communications

Figure 1: Imaging set-up and representative images of the microvascular structures and oxygenation.

From: Large arteriolar component of oxygen delivery implies a safe margin of oxygen supply to cerebral tissue

Figure 1

(a) Multimodal microscopy set-up. Two-photon microscope (TPM) and spectral domain OCT are coupled into the same microscope objective (MO). D1 and D2—dichroic mirrors; EOM—electro-optic modulator; TPM and OCT scanners—galvanometer-mirror-based scanners together with the scan and tube lenses. (b,d) Top views of the two cortical microvascular stacks with the labelled microvascular segments: arterioles (red), capillaries (green) and venules (blue). (c,e) PO2 measurements during normocapnia (c) and hypercapnia (e) overlaid over microvascular structures presented in b and d, respectively. Black squares in b and d represent PO2 measurement fields of view presented in c and e, respectively. In grey-coloured vessels PO2 was not estimated. Scale bars, 200 μm.

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