Figure 1: Sprtn KO causes embryonic lethality.
From: Spartan deficiency causes genomic instability and progeroid phenotypes
(a) Schematic of the mouse Sprtn gene and the targeted alleles. An inverted Neo cassette was inserted in the second intron with flanking FLP recognition target (FRT) sequences. LoxP sites were also inserted at the indicated positions. The floxed and KO alleles were created by crossing heterozygote mice with FLP and Cre-transgenic mice, respectively. Positions of genotyping primers are indicated by arrows. (b) PCR-based genotyping (at weaning) of wild-type and Sprtn heterozygote mice produced by intercrossing Sprtn+/−. (c) PCR-based genotyping of wild-type, heterozygote and KO blastocysts. (d) Blastocysts from Sprtn+/− intercrosses were cultured in vitro and observed by phase-contrast microscopy on 6 consecutive days. Representative images of Sprtn+/+, Sprtn+/− and Sprtn−/− blastocysts are shown. Scale bar, 100 μm.
