Figure 2: Sprtn KO causes impaired cell proliferation and cell death.
From: Spartan deficiency causes genomic instability and progeroid phenotypes
(a) PCR-based genotyping of Sprtn alleles in the indicated MEF lines after 48 h treatment with MeOH or 2 μM 4-OHT. (b) Proliferation of Sprtn-targeted MEFs. Cells treated with MeOH or 4-OHT for 48 h were seeded and cell numbers were counted at the indicated time points. Values are mean±s.d. of three independent experiments. (c) Analyses of apoptosis in Sprtn-targeted MEFs. Two days after the completion of 48 h treatment with MeOH or 4-OHT, cells were stained with Annexin V and PI and analysed by flow cytometry. Values are mean±s.d. of three independent experiments. NS, not significant; ***P<0.001 (two-tailed unpaired t-test). (d) Cell cycle profiling of Sprtn-targeted MEFs. The indicated MEF lines were treated with MeOH or 4-OHT for 48 h. Two days later, cells were stained with PI and analysed by flow cytometry. (e) Cell cycle profiling of SprtnF/F; Cre-ERT2 MEFs (K3) expressing wild-type human Spartan or the E112A mutant after 48 h treatment with MeOH or 4-OHT.
