Figure 7: Premature ageing phenotypes in Sprtn^H/H mice.

(a) Representative images of 12-month-old Sprtn^+/+ and Sprtn^H/H female mice. Note lordokyphosis in Sprtn^H/H indicated by the dotted red line. Scale bar, 1 cm. (b) Representative images of the eyes of Sprtn^+/+ and Sprtn^H/H female mice. Note a cataract in Sprtn^H/H. (c) Body weight, fat mass, body fat percentage and adipose depot weights of 12-month-old Sprtn^+/+ and Sprtn^H/H female mice (n=5, mean±s.d.). Values are normalized to the average of the Sprtn^+/+ mice. Because of the smaller body size of the Sprtn^H/H mice, adipose depot measurements were first calculated relative to body weight. (d) Fat cell diameter measurements (n=5, mean±s.d.). (e) IAT of 12-month-old mice stained for SA-β-gal activity. (f) Relative expression of senescence markers in 12-month-old IAT (n=5, mean±s.d.). Values were normalized to Gapdh and are relative to Sprtn^+/+ IAT. (g) Treadmill exercise ability of 12-month-old Sprtn^+/+ and Sprtn^H/H female mice. Exercise time, distance travelled and workload performed are shown (n=5, mean±s.d.). For all panels, statistical significance is as follows: *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001 (two-tailed unpaired t-test).