Figure 2: Conversion of a peroxisome TA protein into a GET3 substrate.
From: Hydrophobic handoff for direct delivery of peroxisome tail-anchored proteins
(a) The indicated TMD-PEX26 variants were translated in the presence or absence of the indicated recombinant chaperones and GFP was imaged using fluorescence microscopy. Scale bar, 2 μm. (b) Peroxisome targeting of the same TMD-PEX26 variants and chaperone combinations shown in a. Scale bar, 1 μm. This figure is complemented by Supplementary Fig. 7. (c) mRNA encoding the indicated GFP-TMD fusion proteins was microinjected into natural rat kidney cells, which were imaged after several hours of incubation. The upper row shows an overview of the indicated cells. The dotted circles show the position of the nucleus. The dotted box indicates the region that is magnified in the three lower panels in each column. Scale bar, 2 μm. ER (black arrows) was identified by injection of mRNA encoding a mCherry-KDEL fusion protein. Peroxisomes (P, white arrowhead) were labelled by co-injection of purified recombinant mCherry-PTS1. Mitochondria (M, white arrow) were labelled with mitotracker and putative lipid droplets (black arrowhead) were identified by transmitted light microscopy (+TL).
