Figure 1: Identification of ETP-ALL cases with translocations involving the ZEB2 locus and enhanced ZEB2 expression in the immature subtype of human T-ALL patients. | Nature Communications

Figure 1: Identification of ETP-ALL cases with translocations involving the ZEB2 locus and enhanced ZEB2 expression in the immature subtype of human T-ALL patients.

From: ZEB2 drives immature T-cell lymphoblastic leukaemia development via enhanced tumour-initiating potential and IL-7 receptor signalling

Figure 1

(a) Partial karyotype (RHG banding) in case TL88 showing a t(2;14)(q22;q32) translocation. (b) Dual-colour FISH analysis using combination of BCL11B and ZEB2 probes on metaphase spreads from leukaemic cells in case TL88. FISH probe RP11-68I8 is shown in green, RP11-889B13 in red. Scale bar, 10 μm. (c) ZEB2 expression levels in a previously published4 cohort of 64 human paediatric T-ALL cases. Unsupervised hierarchical classification of T-ALL subtypes was based on gene expression and genomic annotations. Mean expression is indicated and Mann–Whitney U-Test used for statistical analysis. (d) ZEB2 expression levels in an independent published cohort of 57 human adult T-ALL cases. Unsupervised hierarchical classification of immature and mature subclasses was based on gene expression and genomic annotations as reported17. Mean expression is indicated and Mann–Whitney U-Test used for statistical analysis. (e) Heatmap showing expression levels of the miRNA-141/200c cluster in a published cohort of human T-ALL patients48. (f) qRT–PCR analysis; relative mRNA expression for ZEB2 and the immature marker genes LYL1, HHEX, MEF2C, PSCD4, FAM46A in six human T-ALL cell lines. Data were presented as average±s.d.

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