Figure 4: Zeb2 overexpression leads to increased leukaemia-initiating potential. | Nature Communications

Figure 4: Zeb2 overexpression leads to increased leukaemia-initiating potential.

From: ZEB2 drives immature T-cell lymphoblastic leukaemia development via enhanced tumour-initiating potential and IL-7 receptor signalling

Figure 4

(a) Quantification of leukaemic stem cell (LSC) frequency in control P53/R26+/+ (n=4) and Zeb2-overexpressing P53/R26-Zeb2tg/+ (n=3) and P53/R26-Zeb2tg/tg (n=3) thymic tumours via serial dilution transplantation of primary tumours cells into immunodeficient NOD/SCID recipient mice. One-way analysis of variance (ANOVA) statistical analysis was used. (b) Secondary leukaemia-initiating capacity was tested for P53/R26-Zeb2+/+ tumours (n=2) vs P53/R26-Zeb2tg/+ (n=1) and P53/R26-Zeb2tg/tg (n=4) tumours via serial dilution transplantation of primary tumour cells into NOD/SCID mice. The cell lines were derived from individual mouse thymic tumours and kept in culture for at least six passages. One-way ANOVA statistical analysis was used. (c) Transplantation of Luciferase-positive LOUCY cells in NSG mice after ZEB2 knockdown (+pLV-TH shZEB2, n=3) resulted in decreased leukaemia development compared with the control cell line (+pLV-TH empty vector, n=3). Student’s t-test was used for statistical analysis. All leukaemia cell transplantation experiments were conducted once.

Back to article page