Figure 4: Hypomethylated regions in Morc1tg/tg correspond to upregulated transposon classes.
From: MORC1 represses transposable elements in the mouse male germline
(a) The overlap of hypomethylated DMRs and control regions (randomly selected regions whose methylation is unaffected by loss of MORC1) with genes and transposon classes is indicated. A DMR or control is counted as overlapping with a feature if there was at least one basepair overlap. (b) Overlap of hypomethylated DMRs with transposon classes upregulated in Morc1tg/tg. Metaplot of methylation over LINE (c) and LTR (d) retrotransposons. BS-seq data were mapped to annotated RepBase consensus sequences for each transposon class and methylation is plotted at each CG in the annotated RepBase consensus sequence relative to the consensus sequence. The first 50 bases from each element, which often have low read coverage, are omitted. As it was usually not possible to determine the orientation of LTR-derived reads relative to LTR transposons, the average methylation level for the relevant LTR species is shown. (e) Global hypomethylation of LTR species corresponding to upregulated transposon classes. Again, BS-seq data were mapped to RepBase consensus sequence for these LTRs.
