Figure 5: Hypomethylated regions in Morc1tg/tg correspond to TSS of transposons active in late embryogenesis.
From: MORC1 represses transposable elements in the mouse male germline
(a) H3K4me3 abundance at E16.5 is calculated within regions identified as hypomethylated DMRs (Hypo DMR) in P2.5 Morc1tg/tg germ cells. (b) Average distributions of uniquely mapping E16.5 RNA-Seq reads (left) and P10.5 RNA-seq reads (right) from individual replicates are plotted over regions identified as hypomethylated at P2.5. (c) Pooled E16.5 ATAC-seq reads are plotted relative to methylation distribution at two loci with hypomethylated DMRs. Each CG is represented as by a bar, with the height of the bar indicating the frequency with which the CG is methylated. A dot at a position indicates no methylation. At least one read must map to the CG for a bar to appear. (d) ATAC-seq reads from individual replicates at E16.5 (left) and P10.5 (right) are plotted relative to DMRs. (e) ATAC-seq read abundance at DMRs and adjacent regions is represented as a boxplot, with each DMR constituting one point in the boxplot. For a–e, DMRs refer to regions hypomethylated in Morc1tg/tg germ cells at P2.5.
