Figure 3: Direct reprogramming of human fibroblasts into iMels.
From: Direct conversion of mouse and human fibroblasts to functional melanocytes by defined factors
(a) Representative flow cytometry plots for analyses of TYR+ and TYRP1+ cells in SMGFPP3-induced cells at indicated time points. Fetal hFs were infected with SMGFPP3, sorted and selected in the medium containing G418. Cells were collected and flow cytometrically analysed at day 0, 40 and 80. (b) Percentage of TYR+ and TYRP1+ cells in SMGFPP3-induced cells at indicated time points. Fetal hFs were infected with SMGFPP3, sorted and selected in media containing G418. Cells were analysed by flow cytometry at day 0, 25, 40 and 80. Representative data are from three independent experiments. (c) Cell morphology of fetal hFs and iMels derived from fetal hFs. Representative images of fetal hFs and iMels derived from fetal hFs (fetal hF-SMGFPP3) were photographed at day 40. Scale bar, 50 μm. (d) Representative flow cytometry plots for analyses of TYR+ cells after cells were reprogrammed by SMGFPP3 by day 100.
