Figure 5: Generation of hiMels using inducible system.
From: Direct conversion of mouse and human fibroblasts to functional melanocytes by defined factors
Human MITF-M, SOX10 and PAX3 were subcloned into doxycycline-inducible viral vectors. Fetal hFs were infected with viruses carrying these three transgenes. (a) qRT–PCR analysis of the transgenic expression of SOX10, MITF and PAX3 in the presence or absence of DOX. (b) qRT–PCR analysis of melanocytic markers in hiMels, which were stopped treating by DOX (DOX withdrawn), hiMels with DOX persistent induction (DOX), hMels and fetal hFs. hMels and fetal hFs here were used as positive and negative controls, respectively. Data shown are mean±s.d. of the expression from three independent experiments. (c–f) Immunostaining analysis of melanocytic markers including TYR (c), DCT (d), TYRP1 (e) and S100 (f) in hiMels that showed no transgenic SOX10, MITF and PAX3 expression without treating by DOX. Scale bar, 30 μm.
