Figure 3: IRF3 is required for constitutive EGFR signalling. | Nature Communications

Figure 3: IRF3 is required for constitutive EGFR signalling.

From: Constitutive and ligand-induced EGFR signalling triggers distinct and mutually exclusive downstream signalling networks

Figure 3

(a,b) U251EGFR cells were transfected with control (scrambled) siRNA or with siRNA directed against IRF3 followed by quantitative real-time PCR for IFIT1 or IFI27. The mRNA levels for both genes are decreased when IRF3 is silenced compared with control siRNA (si Con; unpaired t-test, P<0.0001). (c) Silencing of IRF3 by western blot. In d,e, U251EGFR cells were transfected with IRF3 siRNA (for 72 h) followed by re-expression of IRF3 (or empty vector) and extraction of RNA after 48 h for quantitative PCR for IFIT1 and IFI27. (f) Western blot showing silencing of IRF3 and re-expression in silenced cells. Error bars represent the means±standard deviations of three independent experiments. Data were analysed by one-way ANOVA followed by Tukey’s multiple comparison test. *P: ≤0.05, **P≤0.01, ***P≤0.001.

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