Figure 2: Differential membrane enrichment of acyl and prenyl anchors in OSN ciliary subdomains. | Nature Communications

Figure 2: Differential membrane enrichment of acyl and prenyl anchors in OSN ciliary subdomains.

From: Direct evidence for BBSome-associated intraflagellar transport reveals distinct properties of native mammalian cilia

Figure 2

(a) Representative confocal en face images captured from whole OE transduced with AV- (left) MyrPalm-GFP, (middle) PalmPalm-GFP or (right) GFP-GerGer. Whereas MyrPalm and PalmPalm are present throughout OSN ciliary membranes, GerGer is restricted to within the proximal regions of cilia near the OSN knobs. (b) GerGer-GFP ciliary membrane localization can extend beyond the proximal doublet microtubule segments as revealed by AV-mCherry:Efhc1 expression. Arrows highlight a cilium where GFP-GerGer localization is coincident with mCherry:Efhc1. Arrowheads point to a ciliary segment where GFP-GerGer localizes beyond mCherry:Efhc1. (c) Illustration of an OSN depicting the location of the axon initial segment and of synaptic termini in a glomerulus of the olfactory bulb. (d) Lipid-anchored GFPs freely access OSN axons. Representative confocal images of fixed sections through glomeruli of the olfactory bulb from mice expressing (left) MyrPalm-GFP, (middle) PalmPalm-GFP and (right) GFP-GerGer are shown. Empty circular regions surrounded by DAPI (4',6-diamidino-2-phenylindole; blue)-labelled nuclei represent glomeruli where OSN axons terminate. Scale bars, 10 μm (a); 5 μm (b); 20 μm (d).

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