Figure 6: Peripheral ciliary membrane-associated Arl proteins participate in IFT. | Nature Communications

Figure 6: Peripheral ciliary membrane-associated Arl proteins participate in IFT.

From: Direct evidence for BBSome-associated intraflagellar transport reveals distinct properties of native mammalian cilia

Figure 6

(a–c) Representative live en face confocal images of native OE ectopically co-expressing AV-BBS3:GFP and AV-Arl13b:mCherry. (a) BBS3:GFP is present along OSN ciliary axonemes. Inset shows zoomed image of two axonemes decorated with BBS3:GFP. (b) Zoomed image of the OSN knob showing BBS3:GFP accumulation. (c) Zoomed and colour-shifted image of an OSN ciliary distal tip showing co-localization of BBS3:GFP and Arl13b:mCherry. (d) Line-scan kymogram generated from a TIRFm time series of a BBS3:GFP AV-transduced OSN showing bidirectional movement of BBS3:GFP in an OSN cilium. (e) Line-scan kymogram generated from a TIRFm time series of an Arl13b:mCherry AV-transduced OSN showing particle movement of Arl13b:mCherry in an OSN cilium. (f) Line-scan kymogram of an OSN cilium co-expressing AV-BBS3:GFP and AV-BBS4:mCherry. (g) Line-scan kymogram of an OSN cilium co-expressing AV-Arl13b:mCherry and AV-BBS4:GFP. White arrows highlight a particle possessing both Arl13b:mCherry and BBS4:GFP. Black arrow indicates a group of particles that are labelled by BBS4:GFP but not Arl13b:mCherry. Scale bars, 10 μm (a); 2.5 μm (a (inset), b,c); 5 μm × 10 s (d–g).

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