Figure 4: Sequential assignment of NOESY spectra of VK1 and VK2.
From: A tetrahelical DNA fold adopted by tandem repeats of alternating GGG and GCG tracts
Aromatic regions of 1H NMR spectra (upper panels) and anomeric–aromatic regions of NOESY spectra of VK1 (a) and VK2 (b) constructs at pH 6.0, 0 °C and mixing time of 80 ms. The spectra of VK1 and VK2 were recorded at 2.8 and 1.0 mM oligonucleotide concentration per strand, respectively. Signals of H2 adenine protons are labelled in black. The sequential walks from G1 to A8 and from G9 to G15 are depicted in green and blue, respectively. (b) H2 and H8 resonances of the linker A16 residue are marked in red. The vertical dashed lines indicate resonance frequencies of H8 protons of G15, A8 and A12, which point to distortions in symmetry of VK2.
