Figure 8: Wt1 is important for MDSC function.
From: The Wilms’ tumour suppressor Wt1 is a major regulator of tumour angiogenesis and progression
(a) Ly-6G/Gr-1 immunostaining in B16 and LLC1 tumours and quantification of Ly-6G/Gr-1 signal area density. (b) Morphology of MDSCs sorted from LLC1 tumours evaluated by May-Grünwald-Giemsa staining. (c) Purity of the sorted cells for CD11b+Gr-1+ MDSCs determined by FACS. (d) Quantitative RT–PCR analyses of markers of MDSCs of Wt1 KO MDSCs (derived from LLC1 tumours of Tie2-CreERT2;WT1Lox/Lox mice and tamoxifen treated for 24 h in vitro) relative to control MDSCs (derived from LLC1 tumours of Tie2-CreERT2 mice and tamoxifen treated for 24 h in vitro) (n=3 for each group). (e) Wt1 KO and control MDSCs were co-cultured in triplicates with CFSE-labelled CD3+ T lymphocytes isolated from spleens of non-tumour-bearing animals and proliferation assessed by flow cytometric detection of CFSE dilution after 72 h. Graphs show one representative experiment. (f) Quantification of T-cell proliferation with different T-cell/MDSC co-culture ratios. (n=3). Scale bars, 50 μm. Data are the mean±s.e.m. ***P<0.001.
