Figure 3: Altered ILC populations in the mLN of lymphopenic mice. | Nature Communications

Figure 3: Altered ILC populations in the mLN of lymphopenic mice.

From: CCR7-dependent trafficking of RORγ+ ILCs creates a unique microenvironment within mucosal draining lymph nodes

Figure 3

(a) Detection of ILC populations with flow cytometry in the mLN of WT and Rag−/− mice. (b) Enumeration and (c) percentage of ILC1, ILC2 and ILC3 cells in mLN pool of Rag−/− mice (n=9, 12, 12 in b, 10, 13, 13 in c). (d) Enumeration and (e) percentage of ILC1, ILC2 and ILC3 cells in Rag−/− iLN (n=7, 9, 9). (f) Example of CCR6 and CD4 or T-bet staining on RORγ+ ILCs in mLN of WT and Rag−/− mice. (g) Percentage of RORγ+ ILC3s expressing CCR6, CD4, MHCII, NKp46 and T-bet in Rag−/− and ZAP70−/− mLN (n=7, 6, 13, 10, 4, 6, 13, 10, 10 and 10), pooled from minimum of two independent experiments except MHCII, which is 1. Number of ILC3 (h) and ILC2 (i) cells per individual mLN from WT, Rag−/−, ZAP70−/− and TCRα−/− mice (n=3, 12, 11 and 5). Percentage of ILC population with ILC3 (j) and ILC2 (k) phenotype in mLN from WT, Rag−/−ZAP70−/− and TCRα−/− mice (n=3, 12, 11 and 5). Data pooled from minimum of two independent experiments. Bars represent the median values in all graphs.

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