Figure 5: LTi-like ILC3s require CCR7 to reach the mLN.

(a) Enumeration of the number of ILC1, ILC2, NKp46⁺ and LTi-like ILC3s per individual mLN (n=7 and 9), iLN (n=7 and 5) or spleen (n=7 and 9) of WT and CCR7^−/− mice. Percentage of CD4 T cells, ILC2s, total ILC3s and NKp46⁺ and LTi-like subsets of ILC3s in the mLN (n=6 and 7; b) and spleen (n=7 and 7; c) of bone marrow chimeric mice. Chimeras were generated using CD45.1⁺ CD45.2⁺ hosts, lethally irradiated and reconstituted with CD45.1⁺ WT and either CD45.2⁺ WT (WT:WT) or CD45.2⁺CCR7^−/− (WT:KO) bone marrow. Values shown are a percentage of stated non-host cells only, with residual host CD45.1⁺ CD45.2⁺ cells excluded. (d) Percentage of CD3⁺ CD4⁺ T cells, CD11c⁺ DCs, Lin⁻ IL-7Rα⁺ CCR6⁺ ILC3, Lin⁻ IL-7Rα⁺ CCR6⁻ ICOS⁺ ILC2 or B220⁺ B cells prepared from WT or CCR7^−/− mLN that have migrated towards CCL21 in a transmigration assay (n=4, 2 and 4). *P<0.05, **P<0.01, ***P<0.001 and ****P<0.0001 (Mann–Whitney non-parametric, two-tailed test). Data are pooled from three (a) or two (b–d) independent experiments, bars represent the median values in all graphs.