Figure 5: Tert leads to an increase in Ki67- and pH3-positive cardiomyocytes. | Nature Communications

Figure 5: Tert leads to an increase in Ki67- and pH3-positive cardiomyocytes.

From: Telomerase expression confers cardioprotection in the adult mouse heart after acute myocardial infarction

Figure 5

(a) Representative confocal immunofluorescence images demonstrating co-localization of Ki67 (green) and the cardiomyocyte marker Troponin T (red) in the infarct vicinity. Nuclei stained in blue with DAPI. (IZ, infarct zone; SI, subinfarct zone; CM, cardiomyocyte; nCM, non-cardiomyocyte). Scale bar, 50 μm. (b) Quantification of Ki67-positive cardiomyocytes in the infarct adjacent area given as percentage of total cells. Four to eight fields of three mice each per group were counted (n=3). (c) Representative confocal immunofluorescence images demonstrating co-localization of pH3 (green) and the cardiomyocyte marker Troponin T (red) in the infarct vicinity (CM, cardiomyocyte; nCM, non-cardiomyocyte). DAPI-stained nuclei in blue. Scale bar, 25 μm. (d) Quantification of pH3-positive cardiomyocytes in the infarct adjacent area given as percentage of total cells. Thirty fields per heart and six mice of each group were counted (n=6). Bar graphs show mean values with s.e.m. Statistical analysis: two-sided Student’s t-test, P-values are shown.

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