Figure 5: HCD-induced myeloid cell accumulation is dependent on inflammasome activation in intestinal epithelial cells.
From: Dietary cholesterol directly induces acute inflammasome-dependent intestinal inflammation
(a) Representative flow cytometry plots and quantification of intestinal tissue from adult zebrafish fed control (Hikari) or HCD for 6 h and treated with the active Caspase-1 FLICA substrate. n≥8. (b) Intestinal cells were fixed and double-stained with a cytokeratin antibody to label intestinal epithelial cells. n≥8. (c) Intestinal cells from Tg(lyz:dsRed) adult zebrafish were analysed following HCD and staining with FLICA. n≥5. In all graphs, each dot represents one adult, pooled from two experiments or more. NS, nonsignificant, Error bars are s.e.m. Mann–Whitney test. ***P<0.001 and *P<0.05.
