Figure 1: COP1 deficiency compromises miRNA biogenesis.

(a) Schematic diagram for the DNA/AgNCs-probe based miRNA detection method. (b) Determination of the levels of miR166 and miR172 in WT, hyl1-2 and cop1-6 using the DNA/AgNCs probes. The fluorescent intensity is inversely correlated to the levels of target miRNA. The data were collected as the average value of three biological samples with ±s.d. (n=9). (c) Target sensibility of probes was shown in I₀/I values (I₀ and I are the emission intensity values without and with addition of total RNAs, respectively). The I₀/I intensity versus total RNAs of cop1-6, hyl1-2 and wild type were given. The lower emission intensity represents the higher amount of target miRNA. The data were collected as the average value of three biological samples with ±s.d. (n=9). Significant differences between control and mutant alleles were indicated with asterisks (P<0.0001, student’s t-test). (d) Small RNA blot analyses display the remarkably reduced miRNA levels in cop1-4 and cop1-6. U6snRNA used as loading control. (e) Illumina small RNA sequencing analysis confirmed the highly reduced miRNAs in mutant backgrounds.