Figure 1: Experimental design. | Nature Communications

Figure 1: Experimental design.

From: DNA barcoding reveals diverse growth kinetics of human breast tumour subclones in serially passaged xenografts

Figure 1

Serial passages of individual tumours derived from separate samples of barcoded MDA-MB-231 and SUM-149 cells (nine and four, respectively) are shown in purple, and for cells from patient-derived xenografts in green. White boxes indicate early passages of patient-derived xenografts, before barcoding cells for subsequent clonal analyses. The total number of cells (or fraction of the previous tumour) used to initiate each subsequent tumour, the time before removing the tumour for analysis, and the number and frequency of uniquely barcoded clones (expressed as a proportion of the estimated input number of barcoded cells) are shown on the right. CIC frequencies were calculated as the no. of clones divided by the total no. of barcoded cells transplanted based on the 30% transduction efficiency measured by FACS analysis of input cells.

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