Figure 3: Diverse in vivo clonal growth patterns of human breast cancer cell lines.

(a) Growth patterns of individual clones in primary and secondary derivative tumours generated from M3 cells. In each plot, a separate line portrays the growth activity of an individual clone in successive passages. Clones that remained relatively constant in size between passages are shown in shades of red, and those whose size increased or decreased are shown in shades of yellow and blue, respectively. The area in each plot shaded in grey represents the relative clone size below the threshold used for detecting barcoded clones. In cases where replicate tumours had different limits of detection, and are represented on the same plot, the higher limit is shown. (b) Relative proportions of the different clonal growth patterns at each passage from M3, S3 and S4 cells. Colours in each sector correspond to the colour-coded clonal patterns described in a.