Figure 1: A VRN1-HA fusion accelerates the reproductive development in transgenic barley plants.
From: Direct links between the vernalization response and other key traits of cereal crops
(a) Schematic representation of the VRN1-HA fusion construct, which fuses the VRN1 genomic sequence (minus most of first intron) to the coding sequence for six repeats of the haemagglutinin epitope tag (HA). (b) Homozygous plants carrying the VRN1-HA construct, from line 6 (VRN1-HA(+)-L6) compared with sibling null controls (VRN1-HA(−)−L6) that did not inherit the transgene. Scale bar, 10 cm. (c) The final number of leaves and days to heading of three transgenic lines versus sibling null control plants (n=8). (d) Apex development of VRN1-HA(+)-L6 and VRN1-HA(−)-L6 from the time of first leaf emergence until the fourth leaf emerged. Scale bar, 500 μm. (e) Spike morphology of plants carrying the VRN1-HA construct versus a sibling null. Scale bar, 25 mm. (f) Twenty grains of VRN1-HA(+)-L6 compared with a sibling null control. Scale bar, 1 cm. (g) Grain number per plant and the weight of 100 grains of VRN1-HA(+)-L6 plant versus a sibling null control. Stars indicate statistical significance by Student’s t-test: *P<0.05, **P<0.01, ***P<0.001.
