Figure 2: Detection of VRN1-HA fusion protein in transgenic barley.
From: Direct links between the vernalization response and other key traits of cereal crops
(a) Immunoblot detection of the 6xHA-tagged VRN1 protein using a monoclonal anti-HA antibody in plants carrying the VRN1-HA construct, from three independent lines (6, 14 and 22) descended from independent transformation events, compared with a sibling null control (line 6) and non-transformed wild-type cv. Golden Promise. Relative positions of 40 and 50 kilodalton markers are indicated. (b) Expression level of VRN1 and FT1, relative to ACTIN, in VRN1-HA(+)-L6 compared with a sibling null control. Data are average of three biological replicates, error bar indicates s.e. ***P<0.001 (Student’s t-test).
