Figure 1: EspJ ART domain residues are required for NAD+ binding.
From: The Escherichia coli effector EspJ blocks Src kinase activity via amidation and ADP ribosylation
(a) Overlay of a model of EspJ (cyan) and the canonical ART diphtheria toxin (DT) (orange). The NAD+-binding region is highlighted by a black box and magnified in b (NAD+ C shown in green, O in red, N in blue and P in orange). The side chains of R79, D187 (EspJ) and H21, E148 (diphtheria toxin) are shown (with O in red and N in blue). (c) Two-dimensional 1H–15N HSQC NMR spectra of recombinant 15N-labelled EspJ28-217, EspJ28-217 R79A and EspJ28-217 D187A (black) in the presence of 1 (red) or 10-fold (cyan) molar equivalents of NAD+. Arrows highlight examples of spectral changes upon addition of NAD+. Enlarged boxes show spectral changes in the EspJ28-217 spectra and equivalent peaks in EspJ28-217 R79A and EspJ28-217 D187A spectra.
