Figure 2: iMSD in dilute solution.

Experimental iMSD values at the different timescales for differently sized molecules in dilute solution at 37 °C. Monomeric GFP (N=7 measurements, green dots) shows a linear behaviour in time, as expected for Brownian motion: fit by a free diffusion model (equation 13 in Supplementary Information) yields D_w=134±4 μm² s⁻¹ (, H_r=2.5 nm); Alexa488 (D_w=428±15 μm² s⁻¹, H_r=0.75 nm, , N=7 measurements; blue dots) and 30-nm-diameter fluorescent beads (D_w=22±0.5 μm² s⁻¹, , H_r=15 nm, N=7 measurements; black dots) are acquired under the same conditions. Data are mean values±s.d.