Figure 3: Expression of GFP-tagged FZR-1 and LIN-35 Rb correlates with cell cycle progression.
From: Rb and FZR1/Cdh1 determine CDK4/6-cyclin D requirement in C. elegans and human cancer cells
(a–h) Fluorescence microscopy images of GFP expression and corresponding overlays of fluorescence and DIC images. (a,e) First-stage (L1) larvae showing GFP::FZR-1 (a) and LIN-35::GFP (e) expression. Arrows indicate dividing P cells with high expression levels. Dark spots in the ventral nerve cord are post-mitotic juvenile neurons. (b–d and f–h) Vulval development during the L3 stage. The vulval precursor cells (VPCs) close to the gonad migrate and divide to form the vulva (white boxes). The dividing VPCs express GFP::FZR-1 (box in b,c) and LIN-35::GFP (box in f,g). On completion of vulval formation, expression of FZR-1::GFP and LIN-35::GFP disappears from vulval cells (arrows in d and h). Images illustrate observations of many animals on multiple (>4) days. Scale bars, 10 μm.
